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Primer designs for sequencing amplicons

For spiking-in amplicons into illumina runs

Generates appropriate primers to amplify targets, choose the barcodes wisely, so they don't clash with other libraries (use barcode_caddy !

Specify your customness:

Name Sequence    Notes
FC1

Flow cell A P5

FC2

Flow cell B P7

SP1

Sequencing primer R1

SP2

Sequencing primer R2

BC1

Barcode 1 I7 Index , comma separated, multiple values allowed

BC2

Barcode 2 I5 Index , empty, or same number of entries as in BC1

custom F primer Target specific forward primer
custom R primer Target specific reverse primer

Contact for criticisms/suggestions/bugs

Use of the tool:

Schematics